基于网络药理学、分子对接和实验验证探究蝉花核苷提取物治疗日光性皮炎的作用机制OA

In order to explore the new application and mechanism of action of Cordyceps chanhua nucleoside extracts on solar dermatitis,the targets of C.chanhua nucleoside components treating solar dermatitis were screened by network pharmacology and molecular docking.The results of network pharmacology analysis were verified by mouse animal experiments.The results showed that thymine,adenine,N6-(2 hydroxyethyl)adenosine,inosine and adenosine were the main active nucleoside components of C.chanhua in the treatment of solar dermatitis,and TNF,IL1β,AKT1,IL6,INS,IFNG,EGFR,PTGS2,CTNNB1,and MAPK1 were the core targets of C.chanhua nucleoside components.Enrichment analysis showed that PI3K-Akt,TNF,MAPK and IL-17 signaling pathways were the major biomechanistic pathways of C.chanhua nucleoside components for the treatment of solar dermatitis.Molecular docking analysis showed that there was a strong binding affinity between the main active components of C.chanhua nucleoside components and the core targets.Animal experiments verified that dermal administration of C.chanhua increased collagen content and SOD antioxidant enzyme activity,and decreased PTGS2 content and levels of TNF-ɑ,IL-1β,and IL-17 inflammatory factors in mouse skin tissues.The results of Western blotting showed that C.chanhua nucleoside extracts inhibited the release of p38 MAPK and MMP9,and the molecular mechanism might be related to the MAPK signaling pathway.This study provides a new basis for the development of natural drugs against solar dermatitis.