基于MNP标记的暗褐脉柄牛肝菌菌株鉴定与群体遗传研究OA

Phlebopus portentosus,the first successfully domesticated and cultivated bolete species,has undergone rapid industrial expansion in recent years,highlighting the growing need for accurate strain identification and germplasm protection.In this study,whole-genome resequencing was performed on 66 strains of P.portentosus collected from various regions,resulting in the construction of a database of multiple nucleotide polymorphism(MNP)markers containing 306 loci.Population structure and kinship analyses based on both single nucleotide polymorphisms(SNPs)and MNPs consistently grouped the strains into two distinct genetic clusters.The MNP markers exhibited high resolution and stability across successive subculturing generations,enabling precise strain discrimination.Clonal identification criteria were established using replicate sequencing data of the same strains,with thresholds set at PI_HAT>0.95 and genetic distance(D)<0.01,while a genetic similarity(GS)value of 100%within the MNP dataset was validated as a robust indicator of strain identity.Additionally,machine learning approaches were employed to identify key MNP loci indicative of population divergence,offering valuable markers for varietal authentication and functional genomics.This work established MNP molecular marker database for P.portentosus and validated the potential applications in genetic resource conservation and molecular breeding,providing scientific support for variety protection and germplasm innovation.