芝麻NRAMP家族的全基因组鉴定及其在重金属胁迫下的表达分析OA

Natural resistance-associated macrophage proteins(NRAMP)are a class of metal ion transporters widely present in plants,mainly involved in the transport of divalent metal ions such as Cd2+and Mn2+.To explore the structure and function of the NRAMP family in Sesamum indicum L.,this study identified SiNRAMP family members using bioinfor-matics methods based on sesame whole-genome data,and systematically analyzed their protein and gene structural charac-teristics,as well as expression patterns under heavy metal stress.The results showed that the SiNRAMP family contained a total of six members,distributed on five chromosomes,with amino acid lengths ranging from 447 to 553.All SiNRAMP family members were localized to the cell membrane.The promoter regions of all SiNRAMP genes contained stress-responsive elements and various hormone-responsive ele-ments.The responses of SiNRAMP family members to dif-ferent heavy metal ion stresses were diverse.After 72 h of Cd2+treatment,the expression levels of SiNRAMP2,SiN-RAMP3,SiNRAMP5,and SiNRAMP6 were significantly higher than those in the control group(P<0.05),indicating that these NRAMP genes were collectively involved in the transmembrane transport of heavy metals.In addition,SiNRAMP3 could respond to both Cd2+and Cu2+stresses,suggesting its potential ability to transport multiple heavy metal ions.This study provides a theoretical basis for further elucidating the biological functions of sesame NRAMP genes and their molecular mechanisms in response to heavy metal stress.