花生RLCK-VII亚家族的全基因组鉴定、系统进化与表达模式分析OA

The receptor-like cytoplasmic kinase VII subfamily(RLCK-VII)acts as a key regulator of plant innate immunity,and is also involved in plant developmental processes and abiotic stress responses.In this study,a systematic analysis of the peanut RLCK-VII subfamily was performed to reveal its evolutionary,expressional,and functional characteristics.Phylogenetic analysis divided this subfami-ly into eight clades,and all members contained conserved Motif2 and Motif7,indicating the conservation of their functions.Collinearity analysis showed that this family mainly expanded through chromosomal segment duplication events,which provided a genetic basis for its new functions and phenotypic diversity.Analysis of promoter cis-acting ele-ments revealed that all genes contained light-responsive elements,and abscisic acid-and methyl jasmonate-responsive ele-ments had high frequencies,which was consistent with their functions in stress responses.RLCK-VII-4 and RLCK-VII-70 showed relatively high expression levels in roots and peels.In the late stage of embryonic development,RLCK-VII-29 and RLCK-VII-59 had relatively high expression levels,and these genes were associated with the seed maturation process.Genes such as RLCK-VII-20 and RLCK-VII-58 exhibited relatively high expression levels in flowers and pedicels.The relative ex-pression levels of RLCK-VII-4,RLCK-VII-58,and RLCK-VII-70 in stems were significantly higher than those in roots,leaves,and flowers(P<0.05),while the relative expression levels of RLCK-VII-20 in stems and flowers were significantly higher than those in roots and leaves(P<0.05).Under drought stress,the relative expression levels of multiple genes increased significantly,among which the expression levels of RLCK-VII-26,RLCK-VII-30,RLCK-VII-59,and RLCK-VII-92 increased more than five-fold,and the expression levels of RLCK-VII-52,RLCK-VII-71,RLCK-VII-83,RLCK-VII-87,and RLCK-VII-92 increased more than ten-fold.Brassinosteroid and abscisic acid treatments induced the increased expression of genes such as RLCK-VII-11 and RLCK-VII-5.The relative expression levels of genes including RLCK-VII-1,RLCK-VII-7,and RLCK-VII-22 were not regulated by hormones or abiotic stresses.In conclusion,this study systematically clarified the evolutionary and expression patterns of the peanut RLCK-VII subfamily,which provides a theoretical basis for further under-standing its functions in plant immunity,growth and development,and stress responses.