甘薯IbEFR基因克隆、生物信息学分析及表达模式OA

In this study,the IbEFR gene was cloned from Ipomoea batatas(L.)Lam.using RT-PCR technology.This gene has a full-length coding sequence(CDS)of 3 108 bp,encoding a protein of 1 035 amino acid residues.Domain analysis results showed that the IbEFR protein contained abundant leucine-rich repeat(LRR)sequences and a conserved serine/threonine kinase(S_TKc)domain.Sequence alignment results indicated that the amino acid sequence identity between the IbEFR protein and the Arabidopsis thaliana AtEFR protein was 44.76%.Phylogenetic tree analysis results revealed that the IbEFR gene had the closest genetic relationship with the EFR gene of tobacco.The prediction results of physicochemical properties indicated that the theoretical relative molecular mass of the IbEFR protein was approximately 112 605,and its theoretical isoelectric point was 5.8.In terms of amino acid composition,leucine content was 14.8%,serine con-tent was 10.0%,and glycine content was 8.1%,ranking as the top three,respectively.The instability index was 36.60,so it was determined to be a stable protein.Phosphorylation site prediction results showed that the IbEFR protein had the most serine sites(64),followed by threonine sites(19).Hydrophilicity and hydrophobicity prediction results indicated that IbEFR was a hydrophilic protein.Transmembrane domain prediction results showed that the IbEFR protein contained two obvious transmembrane domains,and the subcellular localiza-tion was most likely in the cytoplasm.Secondary structure prediction results demonstrated that the IbEFR protein was mainly composed of random coils,followed by α-helices and β-sheets.Expression pattern analysis results showed that the expression of the IbEFR gene could be induced and upregulated by abscisic acid(ABA),jasmonic acid(JA)and salicylic acid(SA),indicating that it may be involved in the hormone signal regulation pathway of sweet potato.The results of this study lay a foundation for the subsequent in-depth analysis of the function of the IbEFR gene.