碘帕醇注射液对小鼠睾酮合成与分泌的影响OA

To investigate the effects of iopamidol injection on testosterone synthesis and secretion in mice testicular tis-sues,twenty-five male ICR mice were randomly assigned to the following groups:a control group(C-24)and iopamidol groups at 24 h(I-24),48 h(I-48),72 h(I-72),and 96 h(I-96).Mice in the iopamidol groups received a tail vein injec-tion of 500 μ L iopamidol injection,while the control group received an equal volume of physiological saline.Body weight and behavioral changes were monitored throughout the experimental period.Testosterone concentration in mice serum was detected by ELISA;the expression of genes and proteins of key enzymes of testosterone synthesis(StAR,CYP11A1,3β-HSD)in mice testis was detected by real-time fluorescence quantitative PCR and Western blot;and the changes of testicu-lar tissue morphology in each group of mice were observed by HE staining.The results demonstrated that body weight changes in all treatment groups were not significant,and no obvious abnormalities in behavioral responses were observed.Serum testosterone concentrations decreased significantly at 24 and 48 h after iopamidol injection(P＜0.05)compared with the control group.However,at 72 and 96 h post-injection,serum testosterone concentrations showed a decreasing trend,but the differences were not statistically significant.Similarly,the mRNA and protein expression levels of StAR,CYP11A1,and 3β-HSD were significantly lower than those in the control group at 24 and 48 h after iopamidol injection(P＜0.05).Nevertheless,their expression levels gradually recovered to values close to the control group over time(72 and 96 h).Furthermore,the testicular tissue structure in all treatment groups remained relatively intact,with well-devel-oped seminiferous tubules and tightly ordered arrangement of spermatogenic cells at various stages.Based on the findings above,it can be concluded that the selected dose of iopamidol injection in this study can temporarily reduce testosterone synthesis and secretion in male mice after injection.However,this effect subsides gradually over time.These results pro-vide experimental evidence for the clinical application and risk assessment of iopamidol injection.