树冠覆膜与双层套袋对不知火柑橘果实品质的影响OA

This study compared single-layer bagging plus canopy film-covering(SB+PF)treatment and double-layer bagging(DB)treatment to investigate their effects on fruit quality and molecular mechanisms for cold injury prevention in Citrus reticulata cv.'Buzhihuo'.The results showed that,(1)compared with SB+PF treatment,DB treatment extremely significantly increased single-fruit weight and significantly reduced peel thickness,while extremely significantly increased fruit transverse and longitudinal diameters.SB+PF treatment resulted in significantly higher peel brightness(L* value)compared with DB treatment,but no significant differences were observed in chlorophyll content or carotenoid content.(2)Compared with SB+PF treatment,DB treatment increased fruit titratable acid content significantly,and increased vitamin C content extremely significantly.(3)Transcriptome analysis revealed a total of 133 differentially expressed genes(DEGs)under DB treatment conditions,among which 85 were down-regulated genes and 48 were up-regulated genes.These genes showed enrichment in pathways such as flavonoid biosynthesis and α-linolenic acid metabolism.Through Weighted Gene Co-expression Network Analysis(WGCNA),ten transcription factors related to sugar-acid metabolism were screened out.The study demonstrates that double-layer bagging improves fruit appearance quality by regulating the micro-environ-ment and gene expression.