外源尿囊素对碱胁迫下菜豆种子萌发及抗性的影响OA

To explore the effects of allantoin on seed germination and physiological characteristics of common bean under alkali stress,the alkali-sensitive common bean variety Jinguan was used as the experimental material in this study.Based on the screening of sensitive alkali stress concentration and suitable allantoin soaking concentration,distilled water soaking+distilled water germination culture control(CK),distilled water soaking+40 mmol/L NaHCO3 germination cul-ture treatment(S),2 mmol/L allantoin soaking+40 mmol/L NaHCO3 germination culture treatment(S+All)were set.The contents of soluble protein,soluble sugar,proline and other osmotic adjustment substances in com-mon bean seeds,the activities of antioxidant enzymes such as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),the production rate of superoxide anion free radical(O2·-)and the content of malondialdehyde(MDA)in cotyledons of common bean seeds were meas-ured at 12 h,24 h,36 h,48 h,60 h and 72 h after cul-ture.The results showed that the germination potential and germination rate of seeds treated with 2 mmol/L allantoin were 17.71%and 17.76%higher than those of distilled water treatment,respectively,which alleviated alkaline stress during the germination of common bean seeds.The soluble protein content,soluble sugar content,proline content in common bean seeds,and O2·-production rate and MDA content of the cotyledons of bean seeds treated with S at 12-72 h after culture were generally higher than those of CK,while the antioxidant enzyme activity was generally lower than that of CK in cotyle-dons of common bean.The soluble protein content,soluble sugar content,proline content in common bean seeds and an-tioxidant enzyme activity in cotyledons of common bean of S+All treatment were higher than those of S treatment,while the O2·-production rate and MDA content in cotyledons of common bean were lower than those of S treatment.The results of this study provide a basis for the application of allantoin in bean planting under alkaline stress.