摄食不同饵料对翘嘴鳜脑组织、肝组织中基因表达的影响OA

To investigate the molecular mechanisms underlying the adaptation to formulated feed and dietary transi-tion in carnivorous mandarin fish(Siniperca chuatsi),we performed deep transcriptome sequencing of brain and liver tis-sues from the live-food control group(LFSC)and the formulated-feed treatment group(CFSC).A total of approximately 92.00 Gb of clean data were obtained,with Q20≥98.00%and Q30≥96.00%,and the G+C content ranged from 44.94%to 50.19%.Comparative transcriptomic analysis identified 1 405 differentially expressed genes(DEGs)in the brain(LFSC vs.CFSC),with 486 up-regulated and 919 down-regulated.Similarly,2 850 DEGs were identified in the liver,including 1 298 up-regulated and 1 552 down-regulated.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that liver DEGs were significantly enriched in steroid biosynthesis,fatty-acid degradation,pyruvate metabolism and antigen processing and presentation,whereas brain DEGs were mainly enriched in the cytokine-cytokine receptor interaction pathway.Further functional annotation high-lighted DEGs associated with circadian rhythm(clockb,per1b,RORA),learning/memory(creb1b),retinal photo-transduction(rdh8a,rgra),appetite regulation(tac1,eif4eb)and metabolism(pck1).These genes represent candidate regulators of the species-specific food preference in mandarin fish.The findings provide a theoretical framework for elucida-ting the molecular basis of dietary adaptation and preference shift in mandarin fish,and will facilitate the breeding of novel strains that readily accept formulated feed.