阿如拉-7味散对致病性大肠杆菌感染引起的大鼠肠道损伤的修复作用OA

To investigate the repairing effect of Arula-7 powder(AR)on intestinal injury in rats induced by entero-pathogenic Escherichia coli(EPEC)infection,fifty SD rats were randomly divided into blank control(BC)group,negative control(NC)group,positive control(PC)group,ARH group and ARL group,with ten rats in each group.The BC group received no treatment,whereas the NC,PC,ARH,and ARL groups were intragastrically administered sterile normal saline,0.13 g/kg ciprofloxacin,1.08 g/kg Arula-7 powder,and 0.54 g/kg Arula-7 powder,respectively,and were intraperitoneally injected with EPEC.The results showed that the counts of lactobacilli and bifidobacteria in the cecum of the NC group were significantly lower than those in the BC group,while the counts of enterobacteria and enterococci in the NC group were significantly higher than those in the BC group.The levels of TNF-α,IL-6,and IL-1β in the intestinal tissue of the NC group were significantly higher than those in the BC group,while the IFN-γ level was significantly lower.In the NC group,the ultrastructure of ileal mucosal tight junctions(TJ)was markedly damaged,desmosome(DE)structures were abnormal,and the microvilli ap-peared disordered and fragmented.Compared with the BC group,the relative expression levels of tight junction protein genes Occludin,Claudin-1,and ZO-1 in the NC group were significantly decreased,and severe bacterial translocation was observed.Compared with the NC group,the ARH group showed a significant decrease in the counts of enterobacteria and enterococci,and a significant increase in the counts of lactobacilli and bifidobacteria.The levels of IL-1β,IL-6,and TNF-α in intestinal tissue were significantly reduced,the tight junction structure was markedly improved toward normal,and the relative expression levels of the tight junction protein genes Occludin,Claudin-1,and ZO-1 were significantly upregulated(P<0.05).Additionally,bacterial translocation was noticeably reduced.Therefore,high-dose Arula-7 powder exerts a re-pairing effect on intestinal damage induced by EPEC infection by modulating the gut microbiota structure,improving the tight junction structure of intestinal epithelial cells,and suppressing inflammatory responses.