结直肠癌患者血浆外泌体中circGAPVD1高表达与病情相关OA
Elevated expression of circGAPVD1 in plasma-derived exosomes is correlated with disease progression in colorectal cancer patients
目的 探讨血浆中外泌体来源的circGAPVD1 在结直肠癌(CRC)患者中的差异表达,并分析其作为CRC诊断标志物的潜能.方法 选取 2022 年 1 月至 2023 年 8 月徐州医科大学附属医院经手术治疗且病理确诊为CRC的患者 111 例,选取同期健康检查的 85 名体检者作为对照组,提取血浆中的外泌体,并通过透射电子显微镜、Western blot分析及粒径分析鉴定提取的外泌体,应用RT-qPCR检测circGAPVD1 的表达水平.通过绘制受试者工作特征曲线(ROC)判断circGAPVD1 诊断CRC的效能.结果 与对照组相比,circGAPVD1 在CRC患者血浆外泌体中显著高表达(P＜0.01),circGAPVD1 诊断CRC的灵敏度为 77.5%,特异度为 76.5%.根据患者来源外泌体检测绘制的ROC曲线下面积为0.818 6,提示circGAPVD1 对于CRC有良好的诊断效能.另外,与CRC患者的临床病理资料相联系,高表达的circGAPVD1 与患者的淋巴结转移、临床分期呈正相关.结论 血浆外泌体来源的 circGAPVD1 有望作为CRC的新型诊断标志物.
Objective To explore the differential expression of circGAPVD1 derived from plasma exosomes in pa-tients with colorectal cancer(CRC),and to analyze its potential as a diagnostic marker for colorectal cancer.Methods A total of 111 CRC patients who underwent surgical treatment and were pathologically confirmed at the Affiliated Hospital of Xuzhou Medical University from January 2022 to August 2023 were enrolled,along with 85 healthy individuals from routine physical examinations as controls.Plasma exosomes were isolated and characterized using transmission electron microscopy(TEM),Western blot and nano-particle size analysis.The expression level of circGAPVD1 was detected by RT-qPCR.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the diagnostic efficacy of circGAPVD1 for CRC.Results Compared with healthy controls,circGAPVD1 was significantly expressed higher in the plasma exosomes of patients with CRC(P＜0.01).The sensitivity of circ-GAPVD1 in the diagnosis was 77.5%with specificity as 76.5%.The area under ROC curve was 0.818 6,sugges-ting circGAPVD1 has robust diagnostic efficacy for CRC.In addition,related to the pathological data of patients with CRC,the lymph node metastasis and TNM staging showed a positive relation to high expression of circGAPVD1.Conclusions Plasma exosome-derived circGAPVD1 is a potential novel biomarker which support the clinical diag-nosis of CRC.
詹森;刘宇杰;李天康;张冲;任泽强;张易
徐州医科大学附属医院 胃肠外科,江苏 徐州 221000徐州医科大学附属医院 胃肠外科,江苏 徐州 221000徐州医科大学附属医院 胃肠外科,江苏 徐州 221000徐州医科大学附属医院 胃肠外科,江苏 徐州 221000徐州医科大学附属医院 胃肠外科,江苏 徐州 221000徐州医科大学附属医院 胃肠外科,江苏 徐州 221000
医药卫生
结直肠癌外泌体环状RNA(circRNA)诊断标志物
colorectal cancerexosomecircular RNA(circRNA)diagnostic marker
《基础医学与临床》 2026 (3)
374-380,7
江苏省基础研究计划(自然科学基金)——优秀青年基金(BK20211606)
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