大株红景天注射液对非酒精性脂肪性肝病模型大鼠的治疗作用OA

Objective To investigate the hepato-protective effects of Sorfren injection on rat liver injury resulted from non-alcoholic fatty liver disease(NAFLD).Methods Rats were randomly divided into control,NAFLD model and Sofren-treated groups.The Sofren-treated groups was developed by feeding a high-fat diet(HFD)for 8 weeks.Subsequently,the Sofren-treated group received tail vein injections of Sofren solution(0.4 mL/100 g)for 8 weeks.Serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)levels were measured using an auto-matic biochemical analyzer.Tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6 and malondialdehyde(MDA)were quantified by enzyme-linkedimmunosorbent assay(ELISA).Superoxide dismutase(SOD)activity was assessed by chromogenic method.Histopathological evaluations were performed by Hematoxylin-Eosin(HE)and Prussian blue staining.Immunohistochemistry and Western blot were applied to analyze the expression of glutathione peroxidase 4(GPX4),nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),transferrin receptor 1(TFR1)and ferritin heavy chain 1(FTH1).Results Compared with control group,the model group exhibited significant elevation of serum ALT,AST,MDA,and inflammatory mediators(TNF-α,IL-1β,IL-6),reduction of SOD activity,increased hepatic iron deposition,down-regulated Nrf2,HO-1,and GPX4 expression,and up-regulated level of TFR1 and FTH1(P＜0.05).Treatment with Sofren injection markedly reversed these pathological alterations(P＜0.05).Conclusions Sofren injection may alleviate liver injury in NAFLD rats.