马胃蝇幼虫鉴定方法研究进展OA

Gastrogasteriasis is a severe parasitic disease caused by larvae of the genus Gasterophilus in the family Oestridae parasitizing the gastrointestinal mucosa of equids.In China,the main dominant species causing the disease include Gasterophilus pecorum,Gasterophilus intestinalis,Gasterophilus nasalis,Gasterophilus haemorrhoidalis,Gasterophilus nigricornis and Gasterophilus inermis.As a major horse breeding province,Inner Mongolia Autonomous Region is seriously affected by Gastrogasteriasis disease,which has caused significant economic losses to the horse industry.At present,pathogen identification mainly depends on morphological,molecular biological and serological diagnostic methods.Morphological identification is based on the observation of larval body color and body surface spine arrangement pattern at the macro level,while the micro level focuses on the subtle differences between species with specific ultrastructure.Molecular biology techniques could effectively distinguish the other four species except G.haemorrhoidalis and G.inermis;in serological diagnosis,an indirect ELISA detection method has been established based on the crude antigen and salivary gland antigen of the three-stage Gasterophilus larvae.This paper reviewed the relevant research progress at home and abroad,and summarized the life history and morphological characteristics of the above six dominant species,aiming to provide a reference for the identification of the third-stage larvae of Gasterophilus.