丹夏调斑方抑制氧化低密度脂蛋白诱导的人脐静脉内皮细胞铁死亡的作用研究OA

Objective To investigate the effect of Danxia Tiaoban Decoction on ferroptosis in human umbilical vein endothelial cells(HUVECs)induced by oxidized low-density lipoprotein(ox-LDL),and to reveal to some extent the mechanism of Danxia Tiaoban Decoction in preventing and treating athero-sclerosis(AS).Methods HUVECs were induced by 150 μg/mL ox-LDL to establish an in vitro AS model,which was divided into the blank group,the model group,the negative serum group,the low,medium and high concentration of Danxia Tiaoban Decoction serum groups,and the ferroptosis inhibitor group.Transmission electron microscopy was used to observe the morphology of mitochondria;kits were used to detect the Fe2+content in cells,and flow cytometry was used to detect the apoptosis rate of cells and the levels of intracellular and lipid ROS.Results After induction by ox-LDL,compared with the model group,the levels of Fe2+,intracellular ROS and lipid ROS,and cell survival rate in the Danxia Tiaoban Decoction serum groups and the ferroptosis inhibitor group were significantly increased(P<0.05,P<0.01);compared with the blank group,the mitochondria in the model group were smaller and significantly shrunken under transmission electron microscopy,while the low,medium and high concentration Danxia Tiaoban Decoction serum groups could significantly prevent mitochondrial shrinkage in a dose-dependent manner.Conclusion Ferroptosis occurred in HUVECs induced by ox-LDL,and Danxia Tiaoban Decoction serum could inhibit ferroptosis and exert a protective effect on HUVECs induced by ox-LDL.