基于核因子κB通路探究培补二天膏对肾脏的保护作用机制OA

Objective To investigate the renal protective effect of Peibu Ertian Paste on spontaneously hypertensive rats(SHR)based on the nuclear factor-kappa B(NF-κB)pathway,and to provide a scientific basis for the clinical application of this paste formulation.Methods Ten normotensive Wistar-Kyoto(WKY)rats were selected as the control group.Fifty SHR rats were randomly divided into the model group,the irbesartan group[10.13 mg/(kg·d)],and the low dose group,the medium dose group and the high dose group[0.58,1.15,3.46 g/(kg·d)].After 4 weeks of intervention,tail artery pressure was measured using the non-invasive tail-cuff method.Renal pathological changes were observed via hematoxylin-eosin(HE)staining.The expression levels of inflammatory factors interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)were detected by immunohistochemical staining.The expression levels of key NF-κB pathway proteins,inhibitor of kappa B(IκB)and the nuclear factor p65 subunit(p65),were assessed by western blot.Results After 4 weeks of intervention,compared with the model group,the high-dose Paste group showed a significant decrease in blood pressure(P<0.01),significant improvements in glomerulosclerosis and inflammatory infiltration,reduced IκBα degradation(P<0.01),decreased p65 nuclear translocation(P<0.01),and lowered expression levels of the downstream inflammatory factors IL-6,IL-1β,and TNF-α(P<0.01).The potency of these effects was compared with that in the irbesartan group(P>0.05).Conclusion Peibu Ertian Paste exerts a renal protective effect in SHR rats.The mechanism may involve inhibiting the activation of the NF-κB pathway,reducing IκB degradation and p65 nuclear translocation,thereby down-regulating the expression of inflammatory factors such as IL-6,IL-1β,and TNF-α,and ultimately ameliorating the inflammatory micro-environment in hypertensive renal damage.