低能量激光对高糖介导的巨噬细胞炎性衰老的作用研究OA

Objective Hyperglycemia-associated inflammatory senescence in macrophages is a key factor in inducing macrophage dysfunction and periodontal tissue damage. This study aims to explore the regulatory effects of low-level la-ser therapy (LLLT) on this process. Methods Bone marrow-derived macrophages (iBMDMs) cultured in a glucose-shift medium were subjected to laser treatments for evaluation in terms of phagocytosis,migration,and inflammatory senes-cence levels. Meanwhile,a diabetic mouse model was constructed to compare the effects of insulin monotherapy,LLLT monotherapy,and insulin-combined LLLT on the morphology and inflammatory senescence phenotype of periodontal tis-sues. Results LLLT enhanced cell migration and phagocytosis in iBMDMs,as well as reduced the expression of inflam-matory senescence markers. In the in vivo experiments,LLLT combined with insulin treatment alleviated diabe-tes-induced gingival mucosal epithelial thickening,dis-ordered stratification,shortened epithelial rete pegs,and alveolar bone resorption. Meanwhile,it reduced the in-flammatory senescence burden. Conclusion LLLT ef-fectively reverses hyperglycemia-associated sustained damage to macrophages and periodontal tissues. Thus,it shows strong potential as an effective adjuvant therapy for maintaining oral health in diabetic patients.