p16/Ki-67双染色技术在宫颈病变分流诊断中的价值OA

Cervical cancer is a malignant tumor that seriously threatens women's health,and its occurrence is closely related to persistent infection of high-risk human papilloma virus(HR-HPV).Although the current screening strategies mainly based on cytology and HPV testing have reduced the burden of cervical cancer,the low specificity of HR-HPV testing has led to a large number of transient infected individuals being referred for unnecessary colposcopy,which has become a challenge in clinical practice.The p16/Ki-67 dual staining technique synchronously detects the co-expression of p16INK4a and Ki-67 proteins through immunocytochemical methods,which can objectively identify the state of cell transformation driven by HR-HPV and provides an excellent solution for the triage management of HR-HPV positive individuals.A large amount of evidence shows that for the detection of high-grade cervical intraepithelial neoplasia(CIN2+),the dual staining technique not only has a sensitivity of over 90%,but its specificity(approximately 70%-85%)is significantly better than that of cytological triage.It can reduce colposcopy referrals by about 30%-50%.Moreover,its negative predictive value(>97%)supports extending the follow-up interval for negative individuals,thus optimizing the screening efficiency.The p16/Ki-67 dual staining technique,through accurate risk stratification,significantly reduces overdiagnosis and over-treatment while ensuring the detection rate of lesions.It has become one of the triage tools with the most evidence-based support in the optimization of cervical cancer screening strategies.