自然群体小麦旗叶和株高的全基因组关联分析OA

[Objective]The genetic loci significantly related to flag leaf and plant height in a natural wheat population were investigated to provide the theoretical basis for development of excelllent genes and genetic breeding in wheat.[Method]The genotyping of 300 wheat varieties(lines)was analyzed by using a 120K SNP liquid chip.The flag leaf length(FLL),flag leaf width(FLW)and plant height(PH)of wheat plants in a natural population composed of 300 wheat varieties(lines)were determined under three different environments in 2023 and 2024.The genome-wide association study(GWAS)of flag leaf and plant height was conducted by a mixed linear model(MLM)to screen candidate genes.[Result]A total of 28 SNP loci significantly associated with wheat FLL,FLW,and PH were identified.Among them,four loci associated with flag leaf traits were located on chromsome 2B,4B,5B and 6A,and 24 SNP loci associated with plant height were located on 11 chromosomes such as 1A,1B,1D and so on.For flag leaf length,a gene related to the oxygen-dependent FAD-linked oxidoreductase family was annotated near the locus on chromosome 5B(490 218 405-490 219 967 bp).For flag leaf width,genes encoding cytochrome P450 and a zinc finger protein were annotated near the loci on chromosome 2B(721 411 051-721 413 048 bp)and chromosome 4B(608 452 172-608 452 558 bp),respectively.Among the loci related to plant height,the ribonuclease H protein gene TraesCS4B03G0941800LC was annotated in the 4B chromosome interval(652 574 813-652 575 145 bp),which may be involved in the dwarfing regulation of plant height.[Conclusion]28 key genetic loci related to flag leaf traits and plant height are located,and a new potential gene(TraesCS4B03G0941800LC)related to plant height is identified to provide a new molecular marker for dwarfing breeding in wheat.