48份鲜食葡萄种质遗传多样性的SSR分析OA

[Objective]The genetic diversity level and genetic relationship of 48 table grape germplasm were explored to provide a reference for identification of grape germplasm,conservation of genetic diversity and innovation of grape germplasm resources.[Method]The genetic diversity and genetic structure of 48 table grape germplasms in Yantai region were analyzed by SSR and clustering technology respectively to establish their corresponding DNA finger-print chromatograms and molecular identity cards.[Result]The Shannon's information index,Nei's gene diversity index and polymorphism information content of 9 pairs of SSR primers(A1—A4,B1—B3,C1—C2)with good polymorphism and clear bands selected from 48 table grape germplasm were 2.37,0.86 and 0.85 respectively,which indicated that there were significant genetic differences and abundant genetic diversity among 48 table grape germplasm.The DNA finger-print chromatograms and molecular identity cards of 48 table grape germplasm were successfully constructed.The clustering results showed that Autumn Royal had a significantly distant genetic relationship with the other table grape germplasm.When the genetic distance was 0.05,the other 47 grape germplasm could be divided into two major groups.The groupⅠcould be further divided into 2 subgroups(genetic distance was 0.08),and the group Ⅱ could be further divided into 11 subgroups(genetic distance was 0.10),with three levels of 0.26,0.20,and 0.30 genetic distance.The clustering results were roughly consistent with the classification clustering based on agronomic morphological characteristics and maturity season.[Conclusion]9 pairs of primers with good polymorphism are successfully screened from 48 table grape germplasm.The distinction rate of table grape germplasm reaches 100%based on SSR technology and the SSR molecular fingerprint chromatogram and molecular identification cards of 48 table grape germplasm are successfully constructed.