康欣口服液对衰老大鼠氧化应激和肠道菌群的影响OA

Objective This study preliminarily discussed the anti-aging mechanism of Kangxin Oral Liquid by studying its effects on oxidative stress and intestinal flora in aging rats.Methods The aging rat model was established by D-galactose induction,and Kangxin Oral Liquid was given by gavage at the same time.The body weight was monitored and the indexes of the brain,spleen,liver and kidney were measured.The levels of superoxide dismutase(SOD),malondialdehyde(MDA)and eactive oxygen species(ROS)in serum and organs were measured.The effect of Kangxin oral liquid on the changes in gut microbiota caused by aging was studied by 16S rRNA sequencing.The correlation between intestinal flora and oxidative stress indexes was analyzed.Results Kangxin oral liquid could significantly increase the body weight and organ index of aging rats,reduce the levels of oxidative stress factors in ser-um and organs,increase the abundance of beneficial bacterias such as Pseudoflavonifractor and Ruminococcaceae,reduce the abun-dance of harmful bacterias such as Clostridiales,and maintain the homeostasis of aging-related gut microbiota;the abundance of Clos-tridiales,Pseudoflavonifractor and Ruminococcaceae was significantly correlated with SOD,MDA and ROS levels.Conclusion Kangx-in Oral Liquid can anti-aging by reducing oxidative stress injury and regulating intestinal microbial changes.