新北美圣草苷对牙周炎小鼠牙槽骨吸收和肠道菌群的影响OA

Objective To investigate the inhibitory effect of neoeriocitrin(Neo)on ligature-induced experimental periodontitis in mice and evaluate its biosafety,providing experimental evidence for novel candidate drugs in periodonti-tis treatment.Methods This study has been approved by the Animal Welfare and Ethical Safety Committee.A peri-odontitis model was established in C57BL/6J mice using silk ligation.The mice were divided into control,periodontitis model,and three Neo treatment groups(5,10,20 mg/kg Neo).After 2 weeks of intervention,alveolar bone resorption was analyzed by micro-computed tomography(micro-CT);periodontal tissue pathological changes were observed via hematoxylin-eosin(HE)and Masson staining;osteoclasts were counted using tartrate-resistant acid phosphatase stain-ing;serum inflammatory factor levels[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),in-terleukin-10(IL-10)]were detected by enzyme-linked immunosorbent assay;gut microbiota changes were analyzed by 16S rRNA sequencing;and biosafety was assessed through HE staining of the heart,liver,spleen,lung,kidney,and colon,as well as zonula occludens-1(ZO-1)immunohistochemical staining of the colon.Results Compared with the periodontitis model group,the 10 mg/kg Neo treatment group showed significantly alleviated alveolar bone resorption,manifested as reduced cementoenamel junction to alveolar bone crest distance,increased bone mineral density,bone volume fraction,and trabecular thickness.Additionally,reduced inflammatory cell infiltration,improved collagen fiber arrangement,and a significantly decreased number of osteoclasts were observed in periodontal tissues.Serum levels of pro-inflammatory factors TNF-α and IL-6 were also reduced.Furthermore,10 mg/kg Neo intervention modulated the gut microbiota structure without causing significant multi-organ toxicity or impairing intestinal barrier function.Conclu-sion Neo can effectively mitigate the progression of experimental periodontitis by inhibiting alveolar bone resorption,reducing periodontal tissue inflammation,modulating systemic immunity,and improving gut microbiota.With good bio-safety,Neo is a promising candidate drug for the treatment of periodontitis.