基于序关系-熵权法和正交设计优选化浊散结除痹方水提工艺OA
Optimization of the Aqueous Extraction Process for Huazhuo Sanjie Chubi Decoction Based on G1-Entropy Weight Method and Orthogonal Design
目的 通过序关系(G1)-熵权法和正交设计优选化浊散结除痹方的水提工艺,为其后续成药制备及其临床转化提供依据.方法 采用高效液相色谱法(HPLC)测定化浊散结除痹方溶液中盐酸黄柏碱、异嗪皮啶、落新妇苷、盐酸小檗碱和迷迭香酸含量.以溶液中5种成分含量及出膏率为评价指标,采用G1-熵权法进行主客观组合赋权,获得综合评分(Pj).以Pj为评价依据,通过L9(34)正交试验,考察提取时间、加水量和提取次数对工艺的影响,优选化浊散结除痹方的最佳水提工艺.结果 化浊散结除痹方的最佳水提工艺为加入12倍量水,提取3次,每次2 h.在此工艺下,3批验证实验的Pj相对标准偏差(RSD)为1.0%,且出膏率均＞30%.结论 优选出的化浊散结除痹方最佳水提工艺稳定可行,可为后续该方的成药性研究提供参考.
Objective:To optimize the aqueous extraction process of Huazhuo Sanjie Chubi Decoction using G1-entropy weight method combined with orthogonal design,providing a basis for its subsequent formulation development.Methods:The contents of phellodendrine hydrochloride,isofraxidin,astilbin,berberine hydrochloride,and rosmarinic acid in the extract were determined by high performance liquid chromatography(HPLC).A comprehensive score(Pj)was calculated based on the contents of these five components and the extraction rate using the G1-entropy weight method.With Pj as the evaluation index,an L9(34)orthogonal test was conducted to investigate the effects of extraction time,water addition,and extraction frequency,and to optimize the extrac‐tion process.Results:The optimal extraction conditions were determined as follows:adding 12-fold water,extracting 3 times for 2 h each.In verification tests under these conditions,the relative standard deviation(RSD)of Pj was 1.0%,and the extraction rate was>30%.Conclusion:The optimized extraction process is stable and feasible,providing a reference for the formulation research of Hua‐zhuo Sanjie Chubi Decoction.
郑卓铭;张婷;肖艳;张镇;林逸凡;黄鸣清;苏友新;陈鹏
福建中医药大学第一临床医学院,福建 福州 350004福建中医药大学骨伤学院,福建 福州 350122中医骨伤及运动康复教育部重点实验室,福建 福州 350122福建中医药大学第一临床医学院,福建 福州 350004福建省食品药品质量检验研究院,福建 福州 350001福建中医药大学药学院,福建 福州 350122中医骨伤及运动康复教育部重点实验室,福建 福州 350122福建中医药大学骨伤学院,福建 福州 350122
化浊散结除痹方高效液相色谱序关系-熵权法正交设计提取工艺
Huazhuo Sanjie Chubi Decoctionhigh performance liquid chromatographyG1-entropy weight methodorthogo‐nal designextraction process
《福建中医药》 2026 (2)
16-22,7
国家重点研发计划"中医药现代化"重点专项(2022YFC3501200,2022YFC3501205)国家中医药管理局2021年岐黄学者支持项目(国中医药人教函[2022]6号)福建省省级财政专项(X2022008-财政专项)2023年技术创新重点攻关及产业化项目(2023XQ013)福建中医药大学苏友新岐黄学者传承工作室项目(闽中医[2023]56号)
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