丹龙定喘汤调节Th17/Treg细胞免疫平衡改善OVA诱导的支气管哮喘炎症OA

Objective:To explore the effect of Danlong Dingchuan Decoction(丹龙定喘汤)on ovalbumin(OVA)-induced asthmatic airway inflammation in mice and its underlying mechanism.Methods:Sixty SPF BALB/c female mice were randomly divided into six groups:normal group,model group,dexamethasone group(0.63 mg/kg),and groups receiving Danlong Dingchuan Decoction at low,medium,and high doses(13 g/kg,27 g/kg,and 53 g/kg,respectively),with 10 mice in each group.Except for the normal group,all other groups were sensitized by intraperitoneal injection of 0.2 mL OVA solution on days 1 and 14.On day 28,1%OVA saline solution was administered via ultrasonic nebulization at the maximum output once a day for 30 min over five consecutive days.From day 35 onward,mice were administered with 1%OVA solution via nebulization three times per week to stimulate asthma.Simultaneously,each treatment group received corresponding drugs by gavage once a day for six weeks.One hour after the last administration,the airway hyperrespons-iveness of mice was tested.After detection,the eyeballs were removed to collect blood.A complete blood count(CBC)was performed on whole blood to observe the changes in inflammatory cells.The serum levels of interleukin-10(IL-10)and interleukin-17(IL-17)were detected.Bronchoalveolar lavage fluid(BALF)was collected to detect the levels of Th17 and Treg cells.The right upper lung lobe was fixed with paraformaldehyde,embedded,and sectioned for hematoxy-lin-eosin(HE)staining and Masson's trichrome staining.Results:Compared with the normal group,the model group showed significantly increased Penh values after stimulation with different concentrations of methacholine,significantly el-evated proportion of white blood cells and granulocytes in whole blood,notable peribronchial inflammatory infiltration and collagen deposition,markedly increased proportion of Th17 cells in BALF and serum level of IL-17,and significantly de-creased proportion of Treg cells and serum level of IL-10(P<0.05 or P<0.01).Compared with the model group,all groups treated with Danlong Dingchuan Decoction showed significantly decreased Penh values,markedly reduced propor-tions of white blood cells and granulocytes in whole blood,notably alleviated peribronchial inflammatory infiltration and collagen deposition,markedly decreased Th17 cell proportion in BALF and serum IL-17 levels,and significantly increased Treg cell proportion and serum IL-10 level(P<0.05 or P<0.01).Conclusion:Danlong Dingchuan Decoction can effec-tively improve airway inflammation in OVA-induced asthma model mice,possibly by regulating the immune balance be-tween Th17 and Treg cells,thereby affecting the inflammatory response.