甜菜SSR引物的开发及研究进展OA

As an important sugar crop in China,the sugar beet industry has long faced multiple challenges in its development.Molecular marker-assisted selection technology is key to addressing these challenges,among which simple sequence repeat(SSR)markers play an irreplaceable role in sugar beet genetic improvement due to their various technical advantages.This article aims to systematically review the research progress of SSR markers in sugar beet,with a focus on the evolution of technical development pathways and their practical applications in breeding.The article traces the development strategies of SSR markers from traditional cloning and transcriptome-driven methods to genome-wide high-throughput mining,detailing the transition from genome-based traditional cloning methods,to transcriptome-driven approaches based on expressed sequence tags(ESTs)of functional genes,and further to strategies utilizing high-throughput whole-genome sequencing for systematic mining.It highlights an integrated high-throughput SSR development process based on the Illumina second-generation sequencing platform,combined with bioinformatics tools including fastp(data quality control),BWA-MEM2(sequence alignment),HipSTR(SSR site detection and genotyping),MISA(SSR classification),and Primer3(batch primer design).Furthermore,the article provides a comprehensive summary of the characteristics of developed sugar beet SSR marker libraries and emphasizes the specific applications and outcomes of these markers in germplasm identification,QTL mapping for important traits,molecular-assisted breeding,and variety protection.