纤维素酶和乳酸菌对柠条青贮品质和微生物多样性的影响OA

To investigate the effects of cellulase and lactic acid bacteria on the forage quality and micro-bial community of Caragana korshinskii silage.Fresh C.korshinskii forage was ensiled in PVC vacuum bags for 60 days under three treatments:control(CK,no additive),cellulase supplementation(C,0.1%fresh matter basis),and Lactobacillus plantarum inoculation(L,1×105 cfu/g).Post-ensiling analyses rev-eled that the L group exhibited significantly lower pH and higher concentrations of lactic acid,acetic acid,and propionic acid than both the C and CK groups(P<0.05).The C treatments showed significantly reduced ammonia nitrogen and dry matter contents relative to the control(P<0.05).Both additives treat-ments significantly decreased neutral detergent fiber(NDF)content compared with the control(P<0.05),whereas crude protein(CP)and acid detergent fiber(ADF)contents remained unaffected.Microbial diver-sity(alpha diversity)was significantly reduced in the L treatment(P<0.05),with Lentilactobacillus emerg-ing as the dominant genus.Collectively,Lactobacillus plantarum effectively enhanced silage fermentation quality and reshaped the microbial community structure,offering practical insights for optimizing Caragana korshinskii silage production.