微生物源脂肽型类脂对宁夏地区紫花苜蓿生长的影响及应用研究OA

As microbial metabolites,lipopeptide-type lipids exhibit significant potential in sustainable agriculture.This study conducted a multi-scale experiment approach,including pot,plot,and field trials to investigated the regulatory effects of a Bacillus subtilis-derived lipopeptide-type lipid on alfalfa(Medi-cago sativa)growth and soil nutrients.The results showed that the combined application of lipopeptide-type lipid through seed coating and root irrigation significantly improved the emergence rate of alfalfa from 40.90%in the control group(CK)to 57.70%.Plant height and fresh weight were enhanced by 14.23%and 20.93%,respectively.In the field trials,increases under treatments were found in plant height and fresh weight by 15.31%and 55.88%,respectively,compared with control,while the leaf relative chloro-phyll content(SPAD values)and crude protein content were also promoted by 9.76%and 16.76%,respec-tively.In terms of soil nutrient regulation,treated with fertilizer containing lipopeptide-type lipid signifi-cantly enhanced microbial biomass nitrogen,available phosphorus,and readily available potassium com-pared to the control group.This study demonstrates the dual function of lipopeptide-type lipid in simultane-ously improving alfalfa yield and quality as well as enhancing soil health in semi-arid regions.These find-ings provide a theoretical basis and practical guidance for the application of microbial-derived lipid as green fertilizer and development of sustainable agricultural technologies in ecologically fragile areas.