基于转录组整合分析挖掘蒺藜苜蓿非生物胁迫表观遗传调控基因OA

To further explore the epigenetic regulatory mechanisms underlying Medicago truncatula's response to abiotic stress,this study integrated transcriptome datasets related to salt,drought,low-temperature,and high-temperature stresses from the SRA database.Using WGCNA(weighted gene co-expression network analysis),GSEA(gene set enrichment analysis),and GO(gene ontology)enrichment analysis,we systematically identified epigenetic regulatory genes and their associated biological processes involved in abiotic stresses responses(salt,drought,low temperature,and high temperature).The results showed that 27,234,and 8 core genes related to epigenetic regulation were screened through the three ana-lytical methods,respectively.WGCNA indicated that abiotic stress-responsive genes in M.truncatula were distributed across multiple modules,with 8,5,and 5 core genes in key epigenetic-related modules participating in histone acetylation,methylation,and deacetylation,respectively.The GSEA results indi-cated that the histone methylation and histone acetylation processes constitute the most prominent regula-tory networks under drought and low-temperature stress treatments,respectively.Both histone methyla-tion and histone acetylation constituted the largest regulatory network under high-temperature stress treat-ment,while DNA methylation or demethylation was the largest regulatory network under salt stress treat-ment.From the differentially expressed genes under the four abiotic stresses(low temperature,high tem-perature,salt,and drought),GO enrichment analysis identified 30,76,77,and 88 genes enriched in 42,48,52,and 49 biological processes related to epigenetic regulation,respectively.By integrating the core genes identified through the three analytical approaches,MtrunA17Chr4g0056011 was determined as a common core gene and proposed as a candidate gene for molecular breeding of stress-resistant leguminous forage crops.