芜菁的化学成分研究OA

Objective To investigate the chemical constituents of the taproots of Brassica rapa(Qiamagu)and evaluate their anti-inflammatory activity.Methods The chemical constituents were systematically isolated and purified using silica gel,ODS,Sephadex LH-20 column chromatography,and preparative HPLC.Their structures were elucidated by comprehensive spectroscopic analyses,including NMR,MS,and UV.The cytotoxicity of the isolates against RAW264.7 murine macrophages was assessed using the CCK-8 assay.The in vitro anti-inflammatory activity was evaluated via the Griess method.Results Twenty-five compounds were isolated from the 70%ethanol extract of B.rapa taproots and identified as 2-C-β-D-glucosyl-indole-3-acetonitrile(1),sinapic acid(2),p-coumaric acid(3),ferulic acid(4),methyl sinapate(5),methyl 4-hydroxycinnamate(6),trans-4-methoxycinnamic acid(7),dimethyl feruloyl malate(8),sinapoyl dimethyl malate(9),p-hydroxybenzaldehyde(10),phenylacetic acid(11),phenylethane-1,2-diol(12),3-phenylpropanamide(13),phenylethyl-β-D-glucoside(14),4-(3-hydroxypropyl)phenyl β-D-glucopyranoside(15),idaeusinol B(16),syringaresinol-4-O-β-D-glucoside(17),equiselignan A(18),phaseic acid(19),abscisic acid(20),roseoside(21),bergapten(22),methylthioadenosine(23),5-hydroxymethylfurfural(24),and rabdosia acid B(25).The CCK-8 assay demonstrated the cytotoxicity of compounds 16,22,and 25.To evaluate the anti-inflammatory activity in vitro,the Griess method was employed.The results demonstrated that all tested compounds exhibited varying degrees of inhibitory activity.Conclusion Compound 1 is an indole glucosinolate;compounds 2-9 and 16-18 are phenylpropanoids,compounds 10-12,14 and 15 are benzene ring derivatives,and compounds 13 and 23 are nitrogen-containing compounds.Among them,compounds 1,6-9,13,15,16,18,19,and 23 are isolated from B.rapa for the first time.In the anti-inflammatory assay,with compounds 8 and 9 demonstrating significant effects,yielding IC50 values of 6.95 and 6.46 μmol/L.