基于NF-κB通路探讨生肌止痛洗剂调控肛瘘术后创面修复的分子机制OA
Exploring the Mechanism of Action of Shengji Zhitong Lotion to Promote Postoperative Wound Repair After Anal Fistula Based on NF-κB Pathway
目的:探讨生肌止痛洗剂调控肛瘘术后创伤面修复的作用机制,同时验证其是否通过NF-κB信号通路的调节来影响炎症和血管生长因子表达.方法:选取2024年7月-2025年4月乌鲁木齐市中医医院肛肠科接受手术治疗的120例肛瘘患者,随机分为试验组和对照组,每组患者数量均为60例.试验组采用生肌止痛洗剂联合基础治疗,对照组采用高锰酸钾熏洗联合基础治疗.在术后的第1、7、14天观察两组患者的伤口愈合情况;对肉芽生长情况进行评分;采用ELISA法检测炎症因子TNF-α和IL-6以及血管生长因子VEGF的水平;采用 WB法检测NF-κB p65蛋白的表达量.结果:在第7天和第14天试验组的伤口愈合率显著高于对照组(P<0.05);试验组肉芽生长情况评分显著低于对照组(P<0.05);试验组的IL-6和TNF-α水平显著低于对照组,而VEGF水平高于对照组(P<0.05);试验组的NF-κB p65蛋白表达量低于对照组(P<0.01),并且随着治疗时间的延长,表达量显著下降.结论:生肌止痛洗剂可通过抑制NF-κB通路的激活来降低促炎因子IL-6和TNF-α的表达,同时提高VEGF水平,从而改善创伤的炎症状态,促进血管生成和组织修复,加速肛瘘术后创面的愈合过程.
Objective:Exploring the mechanism by which Shengji Zhitong Lotion regulates wound healing after anal fis-tula surgery,and also to verify whether it affects the expression of inflammation and vascular growth factor through the regulation of NF-κB signaling pathway.Methods:From July 2024 to April 2025,120 anal fistula patients who under-went surgical treatment in the Department of Anus and Intestines of Urumqi Hospital of Traditional Chinese Medicine were randomly divided into the experimental group and the control group,and the number of patients in each group was 60 cases.The experimental group was treated with the combination of muscle-generating analgesic lotion and basic treatment,and the control group was treated with the combination of potassium permanganate fumigation and basic treatment.The wound healing of the two groups was observed on the 1st,7th and 14th postoperative days.The granu-lation growth was scored.The levels of inflammatory factors TNF-α and IL-6 as well as vascular growth factor VEGF were detected by ELISA.The expression of NF-κB p65 protein was detected by WB method.Results:The wound heal-ing rate of the experimental group was significantly higher than that of the control group on days 7 and 14(P<0.05);the granulation growth score of the experimental group was significantly lower than that of the control group(P<0.05);the levels of IL-6 and TNF-α of the experimental group were significantly lower than that of the control group,whereas the level of VEGF was higher than that of the control group(P<0.05);and the expression of NF-κB p65 in the experimental group was lower than that of the control group(P<0.01)and decreased significantly with the exten-sion of the treatment time.Conclusion:Shengji Zhitong Lotion can reduce the expression of pro-inflammatory factors IL-6 and TNF-α by inhibiting the activation of NF-κB pathway,and increase the level of VEGF at the same time,so as to improve the inflammatory state of wounds,promote angiogenesis and tissue repair,and accelerate the healing process of postoperative wounds after anal fistula surgery.
李海玲;肖至君;王京媛;徐晨晨
乌鲁木齐市中医医院,新疆 乌鲁木齐 830000新疆医科大学 研究生院,新疆 乌鲁木齐 830011乌鲁木齐市中医医院,新疆 乌鲁木齐 830000新疆医科大学 研究生院,新疆 乌鲁木齐 830011
医药卫生
NF-κB通路生肌止痛洗剂肛瘘创面修复作用机制
NF-κB Signaling PathwayShengji Zhitong LotionAnal FistulaWound RepairMechanism of Action
《亚太传统医药》 2026 (3)
41-46,6
乌鲁木齐市中医药科技创新项目(ZYYMS-49)
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