胚蛋注射唾液乳杆菌XP132对蛋雏鸡免疫功能及肠道微生物的影响OA

This experiment aimed to investigate the effects of in ovo amniotic cavity injection with Lactobacillus salivarius XP132 on the immune function and intestinal microbiota of layer chicks.A total of 150 SPF laying chicken eggs were selected and randomly divided into two groups,with three replicates per group and 25 eggs per replicate.Group DZ was injected with 0.2 mL of sterile physiological saline into the amniotic cavity at 18 days of embryonation.Group MY was injected with 0.2 mL of a Lactobacillus salivarius XP132 suspension containing 1.21×108 CFU/mL via the amniotic cavity at 18 days of embryonation.The incubation period was 21 days,and the feeding period was 35 days.The results showed that compared to group DZ,serum level of interleukin-2(IL-2)in the group MY was significantly increased at 14,21,and 28 days of age(P<0.05),and significantly increased the levels of interleukin-4(IL-4),interleukin-6(IL-6),and interferon-γ(IFN-γ)at 7,14,and 21 days of age(P<0.05).The number of immunoglobulin A-positive cells(IgA+cells)in the duodenum and ileum at 21 and 35 days of age was significantly increased in group MY(P<0.05),and the number of IgA+cells in the jejunum at 7 and 21 days of age was significantly increased(P<0.05).At 7 and 21 days of age,the Shannon index and Chao1 index in group MY were significantly higher than in the group DZ(P<0.05),and the Simpson index was significantly lower than in the group DZ(P<0.05).At 7 days of age,the relative abundances of Lactobacillus and Oscillospira in group MY were higher than in the group DZ,while the relative abundance of Shigella was lower than in the group DZ.At 21 days of age,the relative abundance of Lactobacillus in group MY was higher than in the group DZ,and the relative abundance of Shigella was lower than in the group DZ.At 35 days of age,the relative abundance of Shigella in group MY was lower than in the group DZ.The study shows that in ovo amniotic injection of Lactobacillus salivarius XP132 can enhance the immune function and improve the diversity of cecal microbiota of layer chicks.