耐寒近芽孢杆菌Rhs-L31的杀线活性及其对松材线虫病的防效OA
Nematicidal activity of Peribacillus frigoritolerans Rhs-L31 on Bursaphelenchus xylophilus and control effect against pine wilt disease
为探究菌株Rhs-L31对松材线虫Bursaphelenchus xylophilus的毒杀活性及其生防潜力,通过形态学与16S rDNA序列分析对菌株进行鉴定,采用菌株发酵菌悬液和发酵滤液处理,测定其对松材线虫的毒杀活性、抑制繁殖能力及其对松材线虫体内脂滴形态的影响,通过盆栽试验评估Rhs-L31发酵菌悬液对油松Pinus tabuliformis苗的防治效果及对油松体内相关防御酶活性的影响.结果表明:菌株Rhs-L31被初步鉴定为耐寒近芽孢杆菌Peribacillus frigoritolerans;Rhs-L31发酵菌悬液和发酵滤液对松材线虫均表现出极强的毒杀活性,处理松材线虫后24 h其校正死亡率均高达100%,分别稀释5倍处理松材线虫后24 h,其校正死亡率分别为76.90%和72.60%;菌株Rhs-L31发酵菌悬液和发酵滤液能显著降低松树线虫繁殖系数和卵数量;油红O染色发现Rhs-L31可破坏松材线虫体内的脂滴形态;Rhs-L31菌悬液注干预处理能显著降低松材线虫病的发生,在处理后14～35 d,防效达52.08%～83.33%;Rhs-L31菌悬液能显著降低油松苗内松材线虫的密度,预防组松材线虫密度显著低于仅接种松材线虫组;菌株Rhs-L31在抵御松材线虫时诱导了油松苗体内多酚氧化酶、过氧化氢酶、超氧化物歧化酶和过氧化物酶的活性增强.研究结果揭示了耐寒近芽孢杆菌Rhs-L31在松材线虫病防控中具有生防潜力,可为松材线虫病高效绿色防控技术的研发提供参考.
To explore the nematocidal activity and biocontrol potential of strain Rhs-L31 against Bursaphelenchus xylophilus,the strain Rhs-L31 was identified by morphology and 16S rDNA sequence analysis.The nematocidal activity,reproductive inhibition ability of the strain Rhs-L31 against B.xylophilus and effect on lipid droplet morphology in B.xylophilus bodies were determined by the treatment of fermentation broth and fermentation filtrate.The control effect of Rhs-L31 fermentation broth on Pinus tabuliformis seedlings and the effects of Rhs-L31 fermentation broth on the activities of related defense enzymes in P.tabuliformis were evaluated by pot experiment.The results showed that strain Rhs-L31 was preliminarily identified as Peribacillus frigoritolerans.Fermentation broth and fermentation filtrate exhibited strong nematocidal activity against B.xylophilus and the corrected mortality was both as high as 100%at 24 h after treatment.The corrected mortality of B.xylophilus was 76.90%and 72.60%at 24 h after treatment with Rhs-L31 fermentation broth and fermentation filtrate diluted five times,respectively.Fermentation broth and fermentation filtrate significantly reduced the reproduction coefficient and egg number of B.xylophilus.Oil red O staining showed that Rhs-L31 fermentation broth and fermentation filtrate could destroy the lipid droplet morphology in B.xylophilus;pot experiments showed that fermentation broth injection treatment could significantly reduce the occurrence of pine wilt disease,and the control effect was 52.08%-83.33%for 14-35 days after treatment.Rhs-L31 fermentation broth could significantly reduce the density of B.xylophilus in P.tabuliformis seedlings,and the density of B.xylophilus in the prevention group was significantly lower than that in the control group.Rhs-L31 enhanced the activities of polyphenol oxidase,catalase,superoxide dismutase and peroxidase in P.tabuliformis seedlings against B.xylophilus.The results revealed that strain Rhs-L31 had potential for biocontrol of pine wilt disease,which could provide a reference for the development of efficient and green prevention and control technology of pine wilt disease.
薛娟娟;梁超琼;王婷;孙孟娇;唐光辉;李培琴
西北农林科技大学林学院,陕西 杨凌 712100陕西省林业科学院,陕西 西安 710016西北农林科技大学林学院,陕西 杨凌 712100西北农林科技大学林学院,陕西 杨凌 712100西北农林科技大学林学院,陕西 杨凌 712100西北农林科技大学林学院,陕西 杨凌 712100
农业科技
松材线虫病生防细菌耐寒近芽孢杆菌杀线活性生防效果
pine wilt diseasebiocontrol bacteriumPeribacillus frigoritoleransnematicidal activitybiocontrol effect
《中国森林病虫》 2026 (1)
1-11,11
陕西省林业科技创新重大专项"松材线虫病绿色防控新技术开发与集成示范"(SXLK2024-0101)陕西省林业科技创新重点专项"松材线虫病的生防菌挖掘及其菌剂研制"(SXLK2023-02-40)
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