菜籽硫苷的低共熔溶剂法提取工艺优化及其抗氧化活性研究OA

Rapeseed glucosinolate has physiological functions such as antioxidation.To extract rape-seed glucosinolate safely and efficiently,the extraction process of rapeseed glucosinolate was optimized through single-factor experiments and response surface experiments based on the selection of appropriate deep eutectic solvent,and the antioxidant activity of rapeseed glucosinolate was evaluated.The results show that the suitable deep eutectic solvent is choline chloride-lactic acid(with a molar ratio of 1∶2),and the op-timal extraction process is a liquid-solid ratio of 38∶1(mL/g),water addition of 29%,extraction time of 31 min,and extraction temperature of 51℃.Under these conditions,the yield of rapeseed glucosinolate is(236.308±11.820)mg/g.Within the range of rapeseed glucosinolate concentration of 0.05-0.25 mg/mL,the rapeseed glucosinolate extracted by choline chloride-lactic acid(with a molar ratio of 1∶2)has good anti-oxidant activity,and the antioxidant activity increases with the increase of rapeseed glucosinolate concen-tration.The scavenging rates of DPPH radicals,ABTS radicals and the reducing power of iron ions were the highest,which were 91.00%,56.00%and 0.600 respectively,higher than those of rapeseed glucosinolate extracted by ethanol solution(with a volume fraction of 70%).