青海省蚕豆赤斑病牡丹葡萄孢病原菌分离鉴定、生物学特性及致病性分化研究OA

To clarify the biological characteristics and pathogenicity of Botrytis paeoniae,the pathogen of broad bean red spot in Qing-hai Province,the target strain was isolated and purified from the lesion tissue by using a tissue separation method.The morphologi-cal observation,molecular biological identification and biological characteristics were used for analysis,and 12 broad bean resourc-es in Qinghai Province were selected for pathogenicity evaluation.Morphological characteristics revealed that the colony edge was regular,the sclerotium was irregularly distributed,and the mycelium was light brown,with a two-branched structure and a dia-phragm.The conidia were mostly oval to rod-shaped,and the conidiophores were round,light brown or nearly transparent.Molecu-lar identification based on ITS and G3PDH gene sequence analysis confirmed the pathogen as Botrytis paeoniae;Biological character-istics indicated that Botrytis paeoniae suffruticosa grew fastest on PDA medium.The optimum carbon source was glucose,and the op-timum nitrogen source was beef extract and potassium nitrate.The optimum pH was neutral(pH 7).Under light conditions,12 h light/12 h dark or 24 h continuous light was more conducive to growth.Pathogenicity differentiation showed that different faba bean resources had different sensitivities to pathogens,and the pathogenic effect on'Yun 122'was the most significant.