不同体型双峰驼血浆外泌体的分离及其蛋白质组学分析OA

In order to investigate the differences in the protein expression of plasma exosomes from two different body types of bactri-an camels,this study used the ultracentrifugation method to isolate plasma exosomes from bactrian camels,transmission electron mi-croscopy(TEM)and nanoparticle tracking analysis(NTA)were used to observe and characterize the morphology of the isolated exo-somes.Based on the proteomics technology,plasma exosomes from wasting body type and normal body type bactrian camel were screened for differentially expressed proteins(DEPs),and bioinformatics analyses such as the GO functional annotation,KEGG sig-naling pathway enrichment analysis,and subcellular localization were performed on the DEPs.The results showed that the bactrian camel plasma exosomes isolated by ultracentrifugation had a cup-shaped or rounded morphology,with a diameter of about 100 nm and an overall size distribution ranging from 30 to 150 nm.A total of 4 903 peptides were labeled using the tandem mass spectrome-try tagging(TMT)combined with the liquid chromatography tandem mass spectrometry(LC-MS/MS),and 172 DEPs were screened out.Bioinformatics analysis showed that these DEPs may be associated with signaling pathways related to lipid metabolism and pro-tein interactions.The results of this study provided a new direction and important information to further explore the biological func-tions of plasma exosomes in bactrian camel.