植物生长调节剂对延胡索产量和品质的影响OA

To screen suitable plant growth regulators for green cultivation of Corydalis yanhusuo W.T.Wang,this study conducted a pot experiment and set up five plant growth regulator treatments:chlormequat 60 mg/L(T1),Bijiu 100 mg/L(T2),uniconazole 50 mg/L(T3),paclobutrazol 150 mg/L(T4),and auxin 30 mg/L(T5).An equal amount of water was used as a control instead of plant growth regulators(CK)to determine the effects of different treatments on the growth,development,yield,and quality of Corydalis yanhusuo W.T.Wang.The results showed that T4 treatment significantly increased the content of photosynthetic pigments in leaves,with chlorophyll a,chlorophyll b,and total chlorophyll content increasing by 30.64%,67.92%,and 37.08%,respectively,compared to CK.In terms of soluble substance content,T3 and T5 treatments significantly increased soluble sugar content by 62.87%and 34.54%,respectively;T5 treatment significantly increased the soluble protein content by 8.04%.In terms of antioxidant enzyme activity,T2 treatment significantly increased catalase(CAT)activity by 93.16%compared to CK treatment;T3 and T4 treatments significantly enhanced superoxide dismutase(SOD)activity,increasing by 51.24%and 56.94%respectively compared to CK;the peroxidase(POD)activity of T1 treatment increased by 32.68%compared to CK;T1,T2,and T4 treatments all significantly reduced malondialdehyde(MDA)content,indicating their positive role in alleviating membrane lipid peroxidation damage.In terms of yield,T5 treatment performed the best,with fresh weight per plant and yield increase rate reaching 18.583 4 g and 392.17%,respectively.In terms of alkaloids,treatments with various plant growth regulators significantly increased the content of berberine,with an increase of 361.46%to 803.94%compared to CK;T5 treatment had the highest content of berberine hydrochloride,which increased by 105.01%compared to CK;T4 treatment had the highest total alkaloid content which increased by 56.04%compared to CK.T4 treatment also significantly increased the total nucleoside content,with a 79.00%increase compared to CK.The correlation analysis results indicated that the fresh and dry weight of tubers were positively correlated with various alkaloids at different significant levels.The residual detection results showed that,except for a slight excess of uniconazole,the residual levels of all other regulators met the national standards.In conclusion,T5 treatment had the best effect on increasing yield,while T4 treatment showed outstanding performance in improving photosynthetic pigments,total alkaloids,and nucleoside content.