基于Th17/Treg免疫平衡探讨艾灸对缺血性脑卒中大鼠脑肠共治机制研究OA

Objective:To observe the effects of moxibustion on Th17/Treg cells in rats with ischemic stroke and to explore its mechanism of improving neurological function through the"brain-gut co-medication"from the perspective of immune homeostasis.Methods:A total of 120 male SD rats were randomly divided into the blank group(25 rats)and the modeling group(95 rats).Among which 75 successful middle cerebral artery occlusion(MCAO)modeled rats were randomly divided into the model group,the probiotic group,and the moxibustion group,with 25 rats in each group.In the probiotic group,gold bifidus was administered by gavage,and in the moxibustion group,moxibustion was administered at the"Baihui"and"Dazhui"points,as well as the bilater-al"Tianshu"and"Zusanli"points,for 20 min each time.Both groups were intervened once a day for 7 consecutive days.At the end of the interventions,the effects of moxibustion on the MCAO rats were detected by the Longa score,TTC staining,H&E stain-ing,ELISA,real-time fluorescence quantitative PCR,Western blot,immunohistochemistry,and flow cytometry.Results:Com-pared to the blank group,the model group exhibited higher Longa scores(P<0.01)and larger cerebral infarct volumes(P<0.01).There was pronounced morphological disruption and inflammatory cell infiltration in the ischemic brain and colon tissues.The level of interleukin-10(IL-10)was decreased(P<0.01),IL-10 and forkhead box P3(FOXP3)mRNA,protein,and posi-tive expressions were reduced(P<0.01).The level of interleukin-17A(IL-17A)was increased(P<0.01),IL-17A and retinoid-related orphan nuclear receptor γt(RORγt)mRNA,protein,and positive expressions were increased(P<0.01).The percentages of CD4+and Treg were decreased(P<0.05),while those of CD8+and Th17 were increased(P<0.05).Compared to the model group,the probiotic and moxibustion groups showed lower Longa scores(P<0.01)and reduced cerebral infarct vol-umes(P<0.01).Tissue morphology in the ischemic brain and colon was relatively intact,and inflammatory cell infiltration was re-duced.The level of IL-10 was increased(P<0.01),IL-10 and FOXP3 mRNA,protein,and positive expressions were elevated(P<0.01).The level of IL-17A was decreased(P<0.01),and IL-17A and RORγt mRNA,protein,and positive expressions were reduced(P<0.01).The percentages of CD4+and Treg were increased(P<0.05),while those of CD8+and Th17 de-creased(P<0.05).Conclusion:Moxibustion ameliorate neurological function damage after ischemic stroke through brain-gut co-treatment,likely by regulating the Th17/Treg immune balance and inhibiting inflammatory responses in the brain-gut axis.