复发性流产患者阴道分泌物的代谢组学分析OA

Objective:To explore the differential metabolites and metabolic pathways associated with vaginal secretions in pa-tients with recurrent spontaneous abortion(RSA).Methods:Using UPLC-Q-Exactive-MS technology,we performed non-targeted metabolomics analysis on vaginal secretion samples from 63 patients with RSA and 47 healthy women,screened for differential metabolites between the two groups with combining multivariate and unitary statistical analysis,and the metabolic path-way analysis of the differential metabolite's was performed,thus finding the relative relationship between the metabolite and RSA.The discriminatory ability of differential metabolites to RSA was also analyzed by subject work characteristic curve(ROC).Re-sults:Eight differential metabolites,including L-proline and L-leucine,and four abnormal metabolic pathways,including arginine and proline metabolism,were screened in the healthy nonpregnant and healthy pregnant groups.Thirteen differential metabolites,including 2,3-Dinor-8-epi-prostaglandin F2α and 3-(2-Naphthyl)-L-alanine,and two abnormal metabolic pathways,including py-rimidine metabolism,were screened in the RSA nonpregnant and healthy nonpregnant groups.Seven differential metabolites,in-cluding glycoursodeoxycholic acid and D-(-)-Ribose,and two abnormal metabolic pathways,including the pentose phosphate pathway,were screened in the RSA pregnancy group and the healthy pregnancy group.Three differential metabolites with area un-der the curve(AUC)>0.7 were identified by ROC curve analysis,Including 2,3-Dinor-8-epi-prostaglandin F2α、3-(2-Naph-thyl)-L-alanine and glycoursodeoxycholic acid.Conclusion:There are differences in the metabolic profiles of vaginal secretions be-tween RSA patients and healthy women.Three metabolites,2,3-Dinor-8-epi-prostaglandin F2α,3-(2-Naphthyl)-L-alanine and glycoursodeoxycholic acid showed excellent discriminatory efficacy for RSA and are expected to serve as potential biomarkers.