南疆3个软籽石榴品种抗寒性比较OA

[Objective]To address the issue of winter freezing injury in soft-seed pomegranates in Southern Xinjiang,this study determined the physiological and biochemical indices related to cold resistance of three main cultivated pomegranate varie-ties,clarified the differences in cold hardiness among the three varieties,and thereby provided a scientific basis for the industri-al layout of the pomegranate industry in Southern Xinjiang.[Methods]Taking three soft-seed pomegranate cultivars,Tunisian Soft-seed,Mule and Black-Seed Sweet,cultivated in Tumushuke as test materials,with their one-year-old dormant branches as experimental samples,an artificial simulated low-temperature environment method was adopted.The changes in physiological and biochemical indices related to cold resistance of the branches of the three soft-seed pomegranate varieties were measured un-der low-temperature conditions of 4℃(CK),0℃,-5℃,-10℃and-15℃.The cold hardiness of the three varieties was comprehensively evaluated by combining the Logistic equation(for LT50 calculation)and the membership function meth-od.[Results]The relative electrical conductivity and malondialdehyde(MDA)content of the one-year-old branches of Mule and Black-Seed Sweet were lower than those of Tunisian Soft-seed.On the contrary,the contents of soluble protein and pro-line,as well as superoxide dismutase(SOD)activity of Tunisian Soft-seed were the lowest,while those of Mule and Black-Seed Sweet were relatively higher.Comprehensive analysis by the membership function method based on multiple physiological indices showed that the order of cold hardiness of the three soft-seed pomegranate varieties from strongest to weakest was:Black-Seed Sweet>Mule>Tunisian Soft-seed.[Conclusion]The results of this study provide important scientific basis and practical guidance for the development of the soft-seed pomegranate industry in Southern Xinjiang.