不同激素配比对吴起楸子试管苗离体生长及扩繁效率的影响研究OA

[Objective]To optimize the in vitro rapid propagation system for Malus prunifolia fromWuqi,this study sys-tematically investigated the regulatory effects of 6-Benzylaminopurine(6-BA)and auxin(IBA)concentration ratios in MS medium on the growth characteristics,propagation efficiency,and physiological indicators of tube plantlets.[Methods]A two-factor completely randomized design was employed,with five concentrations of 6-BA(0.4,0.5,0.6,0.7,0.8 mg/L)and five concentrations of IBA(0.5,0.6,0.7,0.8,0.9 mg/L),forming 10 treatment combinations(T1-1 to T5-2).A hormone-free MS medium served as the control(CK).After 30 days of culture,parameters such as propagation coefficient,plant height,stem diameter,internode length,and chlorophyll content were measured.[Results]The ratio of 6-BA to IBA significantly influenced the growth of tube plantlets.The combination of 0.7 mg/L 6-BA and 0.6 mg/L IBA(T5-2)yielded the highest propagation coefficient(14.125),while 0.5 mg/L 6-BA and 0.9 mg/L IBA(T5-1)resulted in the best per-formance in stem diameter and chlorophyll content.[Conclusion]Principal component analysis indicated that a 6-BA/IBA ra-tio between 1.0 and 1.2(e.g.,1.17)synergistically enhanced proliferation efficiency and physiological indicators,effectively addressing the issue of excessive elongation(internode length<5.0 mm).This study successfully established a high-efficiency,low-cost rapid propagation system,providing a theoretical basis for the large-scale production of Malus prunifolia rootstocks.