核桃青皮提取物对苹果腐烂菌抑制作用研究OA

[Objective]Green husks of mature walnuts were extracted using different extractants,and the inhibitory effect of walnut green husk extracts on Valsa mali(the pathogen causing apple valsa canker)was investigated,so as to provide theo-retical support for the development of bacteriostatic agents based on the reuse of waste walnut green husk resources.[Meth-ods]Taking Valsa mali as the test target,the green husks of mature walnuts were extracted with petroleum ether,ethyl ace-tate,methanol and ethanol at different concentrations(25%,50%,75%,100%).The obtained walnut green husk extracts were then used to determine their inhibitory effects on the mycelial growth of Valsa mali.[Results]The extracts obtained u-sing methanol as the extractant showed the best extraction efficiency for the bacteriostatic components against Valsa mali.The bacteriostatic substances in walnut green husks included both water-soluble and organic solvent-soluble compounds,with the optimal extraction effect achieved when the volumes of water and organic solvent were equal.[Conclusion]The walnut green husk extract obtained by 50%methanol exhibited the strongest inhibitory effect on Valsa mali,with the maximum inhibition rate reaching 88.7%and an EC50 value of 0.223.