基于质谱技术的石决明药材及其制品鉴别研究OA

Objective:To establish a mass spectrometry(MS)identification method for the crude drugs,decoction pieces,standard decoctions and formula granules of Haliotidis Concha,so as to provide a reference for the identification and quality evaluation of Haliotidis Concha.Methods:Ammonium bicarbonate solution was used to extract the proteins and peptides from Haliotidis Concha;the HLB solid-phase extraction column was adopted for impurity removal and enrichment of the target components in the extract.The selected detection ions were m/z 517.27(2⁺)→432.26,m/z 517.27(2⁺)→618.36,m/z 540.30(2⁺)→333.19 and m/z 540.30(2⁺)→819.44.Separation was performed on a Waters BEH C₁₈column(100 mm×2.1 mm,1.7 μm)with acetonitrile and 0.1%formic acid solution as the mobile phase under gradient elution conditions.The flow rate was set at 0.3 mL/min,the column temperature was maintained at 30℃,and the injection volume was 2 μL.Results:The established MS identification method could effectively detect the characteristic ions of Haliotis discus in its crude drugs,decoction pieces,standard decoctions and formula granules.No characteristic ions were detected in samples from other origins and counterfeit products,indicating the strong specificity of this method.Conclusion:The established MS identification method for Haliotis discus is simple and feasible with good applicability,which can provide a scientific reference for the quality research of Haliotidis Concha.