Characterization of the interaction between human cytochrome c and transfer RNApheOA
Cytochrome c(cyt c)is released from mitochondria into the cytosol upon apoptotic stimulation,ultimately triggering programmed cell death.Recent studies have revealed that transfer RNA(tRNA)interacts with cyt c,impeding the formation of the apoptosome complex and thereby suppressing apoptosis.To elucidate the molecular mechanism underlying the interaction between cyt c and tRNA,nuclear magnetic resonance(NMR)-based chemical shift perturbation and intensity analysis were employed to characterize the binding interface between cyt c and tRNAphe.The findings demonstrate that cyt c primarily engages with tRNAphe through its 70–85Ω-loop and N-terminalα-helix.This interaction sterically hinders the accessibility of small molecules,such as H_(2)O_(2),to the hydrophobic pocket of cyt c,consequently attenuating its peroxidase activity.Furthermore,oxidative modification of cyt c,particularly the carbonylation of positively charged lysine residues,weakens this interaction.
Guangqing Zhang;Jianhua Zhan;Xin Chai;Bin Jiang;Maili Liu;Xu Zhang;Danyun Zeng
Key Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,China Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430074,China Optics Valley Laboratory,Wuhan,430074,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,China Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430074,China Optics Valley Laboratory,Wuhan,430074,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,China Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430074,China Optics Valley Laboratory,Wuhan,430074,ChinaKey Laboratory of Magnetic Resonance in Biological Systems,State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Innovation Academy for Precision Measurement of Science and Technology,Chinese Academy of Sciences,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,China
生物科学
InteractionNMRCytochrome ctRNAOxidative modification
《Magnetic Resonance Letters》 2026 (1)
P.1-12,12
financial support from National Key R&D Program of China(2018YFA0704002,2018YFE0202300,2023YFA1607500)National Natural Science Foundation of China(22174152,21991081,2204167,21505153,21675170,2147514621735007,and 22204167)Hubei Provincial Natural Science Foundation of China(2023AFA041)Strategic Priority Research Program of the Chinese Academy of Sciences(XDB0540300)Funding of Wuhan Special Project for Knowledge Innovation(2023020201010085).
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