Protamine-1 encoded recombinant adeno-associated virus for enhanced brain magnetic resonance imagingOA
Magnetic resonance imaging(MRI)is a powerful tool for diagnosing and monitoring brain diseases,but its low sensitivity can hinder early detection.To address this challenge,we utilized chemical exchange saturation transfer(CEST)MRI,which greatly enhances sensitivity for detecting low-concentration compounds.In this study,we developed a CEST contrast agent based on a recombinant adeno-associated viruses(rAAVs)encoding the protamine-1(PRM1)MRI reporter gene.CEST MRI revealed that PRM1 contrast agent effectively highlighted caudate putamen region after injection of the rAAVs into the mouse brain,clearly distinguishing it from the surrounding tissue,with no observable damage.This method provides a sensitive,metal-free CEST contrast agent for in vivo brain cell detection,demonstrating potential for both diagnostic and therapeutic applications in brain diseases.
Kairu Xie;Yaping Yuan;Mou Jiang;Daiqin Chen;Shizhen Chen;Xin Zhou
State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,ChinaState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,ChinaState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,ChinaState Key Laboratory of Magnetic Resonance Spectroscopy and Imaging,National Center for Magnetic Resonance in Wuhan,Wuhan Institute of Physics and Mathematics,Innovation Academy for Precision Measurement Science and Technology,Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics,Huazhong University of Science and Technology,Wuhan,430071,China University of Chinese Academy of Sciences,Beijing,100049,China Key Laboratory of Biomedical Engineering of Hainan Province,School of Biomedical Engineering,Hainan University,Haikou,570228,China
医药卫生
Magnetic resonance imaging(MRI)Chemical exchange saturation transfer(CEST)Protamine 1(PRM1)Recombinant adeno-associated virus(rAAVs)
《Magnetic Resonance Letters》 2026 (1)
P.25-31,7
financially supported by the National Natural Science Foundation of China(82127802,22374157)Strategic Priority Research Program,CAS(XDB0540000,XDC0170000)CAS Youth Interdisciplinary Team(JCTD-2022-13).In addition,Xin Zhou acknowledges the support from the Tencent Foundation through the XPLORER PRIZE.
评论