Time-and dose-related pathological changes in knee osteoarthritis rat model induced by monosodium iodoacetateOA
Knee osteoarthritis(KOA)is a chronic degenerative disease.Monosodium iodoac-etate(MIA)induction is the most commonly used therapeutic effect evaluation and mechanism of action research model;we observed a lack of standardization and uni-formity in current model building methods,which led us to conduct this study.Background:The aim was to investigate the time-and dose-related changes in the behavioral and pathological characteristics in the MIA-induced KOA model rat.Methods:MIA(40,50,and 60 mg/mL)was injected into the left joint of male Sprague-Dawley rats.After 2 weeks,the changes in the KOA rat model were observed by be-havioral evaluation,imaging-level evaluation,and histological-level evaluation.The changes were also compared after 40-mg/mL MIA injection for 2 and 6 weeks.Results:MIA-induced bone surface defects,osteophyte hyperplasia around the artic-ular rim,increased subchondral bone density,thinning of the sparse trabecular bone,structural disorder,and local clustering were observed.The degree of injury gradually increased with the increase in MIA concentration.After 6 weeks,subchondral bone density and sparse trabecular bone increased in the KOA model.Conclusions:The severity of the model also increased significantly with the changes in dose and time.In dose-dependent experiments,this study revealed that 40 mg/mL was the optimal dose to induce significant pathological changes without causing undue discomfort or death in animals.This dose may induce pathological changes stably and is suitable for long-term observation.
Wei Pu;Qi Liu;Shuyan Xue;Siyuan Li;Nan Nan;Yang Liu;Huiqin Hao
Third Clinical College,Shanxi University of Chinese Medicine,Jinzhong,China Basic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,ChinaThird Clinical College,Shanxi University of Chinese Medicine,Jinzhong,China Basic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,ChinaThird Clinical College,Shanxi University of Chinese Medicine,Jinzhong,ChinaThird Clinical College,Shanxi University of Chinese Medicine,Jinzhong,ChinaBasic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,ChinaBasic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,China College of Basic Medical Sciences,Shanxi University of Chinese Medicine,Jinzhong,ChinaBasic Laboratory of Integrated Traditional Chinese and Western Medicine,Shanxi University of Chinese Medicine,Jinzhong,China Engineering Research Center of Cross Innovation for Chinese Traditional Medicine of Shanxi Province,Jinzhong,China College of Basic Medical Sciences,Shanxi University of Chinese Medicine,Jinzhong,China
医药卫生
knee osteoarthritismicro-CTmonosodium iodoacetatepathological change
《Animal Models and Experimental Medicine》 2026 (1)
P.21-30,10
Construction Project of High-Level Traditional Chinese Medicine Key Discipline of National Administration of Traditional Chinese Medicine,Grant/Award Number:zyyzdxk-2023022Key Team of Scientific and Technological Innovation Talents of Shanxi Province with Integrated Traditional Chinese and Western Medicine for Preventing and Treating Rheumatological Diseases,Grant/Award Number:202204051002033Traditional Chinese Medicine+Stem Cell Innovation Project,Grant/Award Number:2024KJZY0062023 Shanxi Graduate Research Practice Project,Grant/Award Number:2023KY6762023 Graduate Innovation and Entrepreneurship Project of Shanxi University of Traditional Chinese Medicine,Grant/Award Number:2023CX023 and 2023CX027Science and Technology Innovation Project for University in Shanxi Province,Grant/Award Number:2022L358Key Laboratory of Rheumatological and Immunological Diseases Treated by Integrated Chinese and Western Medicine,Grant/Award Number:zyyyjs2024021。
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