Optimizing the dengue virus infection mouse model:Comparing different backgrounds and infection route for enhanced stabilityOA
Background:In recent decades,the global incidence of dengue fever has been stead-ily increasing,with continuous geographical expansion.Researchers have successfully modeled most clinical symptoms of human dengue fever using interferon type I(IFN-I)or combined IFN-I/II receptor knockout mice infected with dengue virus(DENV).However,this model requires further optimization to better support related studies.Methods:This study aimed to establish a stable dengue infection model by evaluating the effects of different genetic backgrounds and injection routes on DENV infection in interferon receptor knockout mice.We first infected various strains of interferon receptor-deficient mice with DENV and compared their susceptibility based on clini-cal symptoms,viremia levels,organ indices,histopathological findings,and vascular leakage markers.Subsequently,we selected the most susceptible strain to further investigate the impact of different injection methods on infection outcomes.Results:We found that BALB/c background mice with type 1 interferon recep-tor knockout(IFNAR)had the most obvious symptoms.Subsequently,we selected IFNAR−/−BALB/c mice to further explore the effects of different injection methods on dengue virus infection.The results showed that the intraperitoneal injection group had the most severe clinical symptoms,the longest duration of viremia,and the most obvious degree of organ damage.Conclusion:Through systematic screening and optimization,we established a robust animal model of dengue virus infection via intraperitoneal injection in IFNAR−/−BALB/c mice.This model offers a valuable tool for future dengue research.
Dan Liao;Ming Zhong;Wenjiang Zheng;Zhendong Guo;Ye Zhou;Qiuhong Li;Lijuan Qiu;Liangwen Yu;Haishan Long;Geng Li
Chinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,China Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,ChinaExperimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China Zhongshan Hospital of the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Zhongshan,ChinaThe First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China Guangdong Clinical Research Academy of Chinese Medicine,Guangzhou,China Guangdong Engineering Research Center of Commercialization of Medical Institution Preparations and Traditional Chinese Medicines,Guangzhou,China Guangdong Engineering Technology Research Center of Commercialization of Lingnan Special Medical Institution Preparations,Guangzhou,ChinaGuangdong Provincial Hospital of Traditional Chinese Medicine,Guangzhou,ChinaExperimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,ChinaExperimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,ChinaExperimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,ChinaChinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,ChinaExperimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,ChinaChinese Medicine Guangdong Laboratory,Guangzhou University of Chinese Medicine,Zhuhai,China Experimental Animal Center,Guangzhou University of Chinese Medicine,Guangzhou,China State Key Laboratory of Traditional Chinese Medicine Syndrome,Guangzhou University of Chinese Medicine,Guangzhou,China
医药卫生
dengue feverIFNAR−/−BALB/c miceinterferon receptor knockoutmouse model
《Animal Models and Experimental Medicine》 2026 (1)
P.103-114,12
Guangdong Province Medical Research Fund Project,Grant/Award Number:B2024112The Scientific Research Special Project of the Joint Construction Project of High-level Hospitals between Guangzhou University of Chinese Medicine and the Scientific Research Fund Project,Grant/Award Number:GZYZS2024G09Special Project of the Research Platform of Guangdong Provincial Department of Traditional Chinese Medicine,Grant/Award Number:20254040the Project of the Incubation Program for the Science and Technology Development of Chinese Medicine Guangdong Laboratory/Hengqin Laboratory,Grant/Award Number:HQL2024PZ043Guangdong Province Natural Science Foundation-Guangzhou-South China Joint Youth Fund Project,Grant/Award Number:2023A1515110849。
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