Evaluation of the performance of a new serum amyloid A chemiluminescence immunoassayOA
Objective:Serum amyloid A(SAA)is a protein involved in the acute phase of inflammation.SAA expression is upregulated in humans during the acute phase of various viral infections;in addition,SAA can be a useful biomarker to predict the severity and prognosis of COVID-19 patients.This study aimed to evaluate a new chemiluminescence test for SAA detection.Methods:All serum samples were measured for SAA on a Maglumi 800(Snibe,Shenzhen,China)and compared with a BN ProSpec(Siemens,Munich,Germany)in the routine of the clinical laboratory of the University Hospital of the Tor Vergata University of Rome(Rome,Italy).Analytical precision,the correlation coefficient,and linearity were assessed.Statistical analyses were performed.Results:The linearity test was performed via serial dilutions and revealed a correlation coefficient equivalent to 0.9998.The results of the Snibe SAA test correlated well with those obtained by the SAA Siemens test,with a correlation coefficient of 0.974(P<0.001).The intraand interrun precision,as well as carryover,were assessed.-Conclusions:The results obtained from this study demonstrated that the new Snibe SAA test has reliable analytical performance and good accuracy and could represent a valid tool for routine hospital laboratory analysis.
Martina Pelagalli;Flaminia Tomassetti;Serena Sarubbi;Eleonora Nicolai;Alfredo Giovannelli;Francesco Turis;Camilla Illuminati;Maria Morello;Cinzia Callà;Alessandro Terrinoni;Sergio Bernardini;Massimo Pieri
Department of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyUniCamillus-Saint Camillus International University of Health and Medical Sciences,Rome 00131,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory and Infectious Sciences,Fondazione Policlinico Universitario“A.Gemelli”IRCCS,Università Cattolica del Sacro Cuore,Rome 00168,ItalyDepartment of Laboratory and Infectious Sciences,Fondazione Policlinico Universitario“A.Gemelli”IRCCS,Università Cattolica del Sacro Cuore,Rome 00168,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory and Infectious Sciences,Fondazione Policlinico Universitario“A.Gemelli”IRCCS,Università Cattolica del Sacro Cuore,Rome 00168,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,ItalyDepartment of Laboratory Medicine,Tor Vergata University Hospital,Rome 00133,Italy Department of Experimental Medicine,University of Tor Vergata,Rome 00133,Italy
医药卫生
Serum amyloid A(SAA)InflammationMethod validationAutomationClinical laboratoryC-reactive protein(CRP)Erythrocyte sedimentation rate(ESR)
《LabMed Discovery》 2025 (3)
P.16-21,6
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