A Rh complex anchored cathode for cofactor regeneration and asymmetric reduction coupled with immobilized enzymesOA
Chiral compounds have a huge market demand in the fields of pharmaceuticals,pesticides,and fine chemicals.Enzymatic electrosynthesis can couple enzyme catalysis,possessing high product purity,high efficiency,and mild conditions,with electrochemical regeneration of expensive cofactor nicotinamide adenine dinucleotide(NADH),possessing easy process monitoring and simple operation for efficient chiral synthesis.In this study,hydrophobic covalent organic framework(COF)was synthesized as the immobilized carrier,which not only enhanced the enzyme catalysis through enriching substrate but also enhanced the stability and reuse of the enzyme.Besides,Rh complex was anchored on hydrophilically-modified electrode to promote the regeneration of NADH,where the anchor of Rh complex can effectively avoid the mutual deactivation from the interference between electron mediator and enzyme,and simplify the separation of products.The immobilized enzyme catalysis and the electrochemical cofactor regeneration were coupled to construct an enzymatic electrosynthesis system for the efficient asymmetric reduction to obtain chiral alcohols,with a maximum turnover frequency(TOF)of 101.1 h^(-1).Furthermore,the relevant parameters of the system were optimized,and the substrate scope was expanded.
Yaoxuan Li;Quan Yuan;Hao Zhao;Guanhua Liu;Xiaoyang Yue;Yunting Liu;Yanjun Jiang
School of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,China National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin 300130,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,China National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin 300130,ChinaSchool of Chemical Engineering and Technology,Hebei University of Technology,Tianjin 300401,China National-Local Joint Engineering Laboratory for Energy Conservation in Chemical Process Integration and Resources Utilization,Hebei University of Technology,Tianjin 300130,China
化学化工
enzymatic electrosynthesisasymmetric reductionimmobilized enzymenicotinamide adenine dinucleotide(NADH)regenerationelectron mediator
《Nano Research》 2026 (1)
P.268-277,10
supported by the National Key Research and Development Program of China(No.2023YFA0914500)the National Natural Science Foundation of China(Nos.22378096,22308083,and 22178083)the Natural Science Foundation of Hebei Province(No.B2022202014)the Educational Commission of Hebei Province(No.JZX2023012).
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