Unique Sertoli cell adaptations support enhanced spermatogenesis in chickensOA
Background The cellular basis of testicular development and spermatogenesis for the extreme sperm density in chickens(100-fold higher than mammals)remains poorly defined.A comprehensive understanding of the molecular characteristics driving poultry testicular development is crucial for explaining this enhanced spermatogenic capacity.Results Here,we first established a single-cell transcriptome profile of chicken testes from hatching to maturity,identifying the dynamic transcriptional characteristics of germ cell fate transition and exploring the developmental characteristics of Sertoli cells and Leydig cells.Multi-species comparisons revealed a higher proportion of germ cells and the unique adaptations of Sertoli cells in chicken testes.Most importantly,our results demonstrated that Sertoli cells dominated in somatic composition of mature chicken testes,and proliferating Sertoli cells persisted in chicken testes even after sexual maturity,while no proliferating Sertoli cells in mammals.We also found a richer interaction network between chicken testicular cells,especially the specific activation of Sertoli cell interaction signals,such as TGF-β,BMP,EGF,and activin.These adaptations of Sertoli cells may support the spermatogenic superiority in chickens.Additionally,our results indicated that cAMP responsive element binding protein 5(CREB5)played a crucial role in maintaining the maturation and function of chicken Sertoli cells,and circadian rhythm promoted testosterone secretion and the development of Leydig cells.Conclusion Our study revealed that the sustained proliferative capacity of Sertoli cells,their enriched signaling network,and the regulatory roles of CREB5 and circadian rhythms collectively represented unique testicular adaptations in chickens.These findings may hold extraordinary significance in understanding the molecular characteristics of poultry testicular development,and provide a plausible framework for explaining enhanced spermatogenesis in poultry.
Gaoqing Xu;Zhuoxuan Gu;Ziming Wang;Jing Zhao;He Ding;Hongyu Liu;Yi Fang;Xin Ma;Jing Guo;Wenfa Lyu;Jun Wang
College of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China College of Animal Science,Anhui Science and Technology University,Chuzhou 233100,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,ChinaCollege of Animal Science and Technology,Jilin Agricultural University,2888 Xincheng Street,Changchun,Jilin Province 130118,China Key Laboratory of Animal Production,Product Quality and Security,Ministry of Education,Jilin Agricultural University,Changchun,Jilin Province,China
农业科技
Chicken testesLeydig cellsSertoli cellsSingle-cell RNA sequencingSpermatogenesis
《Journal of Animal Science and Biotechnology》 2026 (1)
P.192-212,21
supported by“National Key Research and Development Program of China”(Grant No.2021YFF1000701)“National Natural Science Foundation of China”(Grant No.U22A20509)。
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