Targeted mutagenesis of FATTY ACID ELONGASE 1 entails near complete elimination of very long chain fatty acids in the seeds of camelina cultivar LigenaOA
Genome editing has the potential to enhance yield and quality traits of crops.However,standard genetic transformation methods are not always applicable to modern germplasm.To tackle this challenge in the widely cultivated variety Ligena of the oilseed crop camelina(Camelina sativa(L.)Crantz),an only recently established principle of adventitious shoot formation from immature zygotic embryos was employed to further improve its fatty acid profile.In this approach,the three subgenomic homeologs of the FATTY ACID ELONGASE 1(FAE1)gene were subjected to targeted mutagenesis.To pre-validate the Cas9-interacting,target motif-specific guide(g)RNAs,a robust protoplast-based DNA transfection method was established.This assay demonstrated that the preselected gRNAs were capable of eliciting mutations across all three camelina FAE1 homeologs.Likewise,targeted mutagenesis was successful at the whole-plant level.Triple-homozygous fae1 knockout mutants were identified amongst a segregating generation M_(3) family.Gas chromatography of lipid extracts from M4 seeds revealed a significant increase in all unsaturated C18 fatty acids including the particularly valuable a-linolenic acid.This was accompanied by a near elimination of the C20 and C22 very long-chain fatty acids including the nutritionally problematic erucic acid.Altogether,we have developed camelina elite lines with two significantly improved properties of high relevance for a health-promoting human nutrition.
Barno Ruzimurodovna Rezaeva;Amélie A.Kelly;Martin Fulda;Ingrid Otto;Iris Hoffie;Sindy Chamas;Ivo Feussner;Jochen Kumlehn
Plant Reproductive Biology,Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Seeland,GermanyAlbrecht-Von-Haller Institute for Plant Sciences and Göttingen Center for Molecular Biosciences(GZMB),Department of Plant Biochemistry,University of Göttingen,Justus-Von-Liebig-Weg 11,Göttingen,GermanyAlbrecht-Von-Haller Institute for Plant Sciences and Göttingen Center for Molecular Biosciences(GZMB),Department of Plant Biochemistry,University of Göttingen,Justus-Von-Liebig-Weg 11,Göttingen,GermanyPlant Reproductive Biology,Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Seeland,GermanyPlant Reproductive Biology,Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Seeland,GermanyPlant Reproductive Biology,Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Seeland,Germany Research Centre for Horticultural Crops,Kühnhäuser Straße 101,Erfurt,GermanyAlbrecht-Von-Haller Institute for Plant Sciences and Göttingen Center for Molecular Biosciences(GZMB),Department of Plant Biochemistry,University of Göttingen,Justus-Von-Liebig-Weg 11,Göttingen,GermanyPlant Reproductive Biology,Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)Gatersleben,Corrensstraße 3,06466 Seeland,Germany
农业科技
Adventitious shootsImmature embryoTargeted mutagenesisVery-long-chain fatty acidsErucic acid
《aBIOTECH》 2025 (4)
P.816-830,15
funded by the German Federal Ministry of Education and Research(BMBF),grant FKZ031B0343A and Cthe German Research Foundation(Deutsche Forschungsgemeinschaft)grant 491250510.
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