Targeted mutagenesis of SlGAD3 generates very high levels of GABA in commercial tomato cultivarsOA
GABA,a non-proteinogenic amino acid with anti-hypertensive properties,holds health-beneficial potential when enriched in crops.Previous studies have established that targeted disruption of the calmodulin-binding domain(CaMBD)of the tomato glutamate decarboxylase 3(SlGAD3)enhances GABA biosynthesis.In this study,we used CRISPR/Cas9-mediated gene editing to precisely modify the CaMBD coding sequence of SlGAD3 in three elite tomato varieties(SFT1,SFT2,and SFT3).Under our experimental conditions,targeted editing of SlGAD3 led to substantial accumulation of GABA in all three varieties without compromising key agronomic traits such as fruit size and number.Although flowering was delayed in SFT2 and SFT3 mutants,SFT1 mutants had higher GABA levels but also maintained a wild-type flowering time.This result highlights the critical importance of selecting specific varieties,such as SFT1,to minimize pleiotropic effects.By identifying varieties that can accumulate high levels of GABA without major reductions in growth and yield potential,this work bridges a critical gap between plant metabolic-engineering research and practical applications in commercial crop-improvement programs.
Lei Zhu;Shiyang Zhang;Qingfeng Niu;Yansha Li;Xiaomu Niu;Pengcheng Wang;Jian-Kang Zhu;Zhaobo Lang
Institute of Advanced Biotechnology,Institute of Homeostatic Medicine,School of Medicine,Southern University of Science and Technology,Shenzhen 518055,ChinaInstitute of Advanced Biotechnology,Institute of Homeostatic Medicine,School of Medicine,Southern University of Science and Technology,Shenzhen 518055,China Shandong Shunfeng Biotechnology Co.Ltd.,Jinan 250000,ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding,School of Life Sciences,Anhui Agricultural University,Hefei 230036,ChinaShandong Shunfeng Biotechnology Co.Ltd.,Jinan 250000,ChinaShandong Shunfeng Biotechnology Co.Ltd.,Jinan 250000,ChinaInstitute of Advanced Biotechnology,Institute of Homeostatic Medicine,School of Medicine,Southern University of Science and Technology,Shenzhen 518055,ChinaInstitute of Advanced Biotechnology,Institute of Homeostatic Medicine,School of Medicine,Southern University of Science and Technology,Shenzhen 518055,ChinaInstitute of Advanced Biotechnology,Institute of Homeostatic Medicine,School of Medicine,Southern University of Science and Technology,Shenzhen 518055,China
农业科技
Gamma-aminobutyric acidTomatoGene editingGlutamate decarboxylase
《aBIOTECH》 2025 (4)
P.693-697,5
supported by the National Key R&D Program of China(grant numbers:2022YFD2100101 and 2021YFA1300400)the National Natural Science Foundation of China(grant numbers:31671735 and 31970516)the Guangdong S&T Program(grant number:2024B1111130001)the Shenzhen Science and Technology Program(grant numbers:JCYJ20241202125311016 and KQTD20240729102038044)the Shandong Province Key R&D Program(Competitive Innovation Platform)(2023CXPT095)Shandong Shunfeng Biotechnology Co.Ltd.,Jinan,China.
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